Restricting Promiscuity of Plant Flavonoid 3'-Hydroxylase and 4'-O-Methyltransferase Improves the Biosynthesis of (2S)-Hesperetin in E. coli

Liu, Juan; Xiao, Zhiqiang; Zhang, Siqi; Wang, Zhen; Shan, Yang; Liu, Juan; Xiao, Zhiqiang; Zhang, Siqi; Wang, Zhen; Shan, Yang; Liu, Juan; Xiao, Zhiqiang; Zhang, Siqi; Wang, Zhen; Shan, Yang; Liu, Juan; Chen, Yun; SCI

机构： Hunan Univ, Coll Biol, Longping Branch, Changsha 410125, Peoples R China; Hunan Acad Agr Sci, Agr Prod Proc Inst, Changsha 410125, Peoples R China; Hunan Key Lab Fruits & Vegetables Storage Proc Qua, Hunan Agr Prod Proc Inst, Changsha 410125, Peoples R China; Chalmers Univ Technol, Dept Life Sci, SE-41296 Gothenburg, Sweden;

关键词： Enzyme promiscuity; flavonoid; (2S)-hesperetin; directed evolution; flavonoid 3'-hydroxylase; flavonoid 4'-O-methyltransferase

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